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2022 Vol.55, Issue 4 Preview Page

Original research article

Long-Term Inorganic and Organic Fertilizations Affect CH4 Oxidation Potential and Methanotrophic Community Structure in Paddy Soils
Sohee Yoon1
,
Juhee Lee1
,
Yeomyeong Lee1
,
Hyerin An1
,
Ki Youl Jung2
,
Seong Hwan Oh2
,
Chang Hoon Lee3*
,
Sang Yoon Kim4*
1Graduate Student, Department of Agricultural Chemistry & Interdisciplinary Program in IT-Bio Convergence System, Sunchon National University, Suncheon 57922, Korea
2Senior Researcher, Department of Southern Area Crop Science, National Institute of Crop Science, Miryang 50424, Korea
3Professor, Department of Fruit Science, Korea National University of Agriculture and Fisheries, Jeonju 54874, Republic of Korea
4Professor, Department of Agricultural Chemistry & Interdisciplinary Program in IT-Bio Convergence System, Sunchon National University, Suncheon 57922, Korea
*Corresponding Author
30 November 2022. pp. 475-487
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Abstract

Long-term fertilizations of inorganic and organic fertilizers can affect microbial abundance and community structure in agricultural soils, particularly controlling the activities of methane (CH4) microbes such as methanotrophs in the rice paddy soils. However, the effect of fertilizations on CH4 oxidation potential and methanotrophic community remains unclear. In order to investigate the response of the soil bacterial community abundance, composition, and CH4 oxidation potential under four different fertilization regimes (control, NPK, compost, and NPK + compost) in a 49-year old paddy field, the soil bacterial community abundance and structure including CH4-oxidizing microbes as well as the potential of CH4 oxidation were investigated by assessing real time quantitative PCR (qPCR), Miseq illumina sequencing analysis based on 16S rRNA genes, and oxic in vitro slurry incubation, respectively. Long-term fertilizations changed significantly soil biochemical characteristics, mainly influencing carbon and nitrogen pools in rice paddy soils. CH4 oxidation potential was stimulated by inorganic fertilizations, mainly increasing the relative abundance of the genus Methylosarcina probably due to increased N availability and soil pH in the soils. Our results showed inorganic fertilizations may enhance CH4 oxidation potential by altering methanotrophic communities, which potentially mitigate CH4 emissions in paddy ecosystems during the cultivation. However, additional research would be necessary for a better understanding of CH4 dynamics in rice paddy soils under the long-term field conditions.

CH4 oxidation potential during slurry incubation under the different fertilization regimes.
Keywords
Inorganic fertilizer
Long-term fertilization
Methane oxidation
Methanotrophic composition
Organic fertilizer
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Information
  • Publisher :Korean Society of Soil Science and Fertilizer
  • Publisher(Ko) :한국토양비료학회
  • Journal Title :Korean Journal of Soil Science and Fertilizer
  • Journal Title(Ko) :한국토양비료학회 학회지
  • Volume : 55
  • No :4
  • Pages :475-487
  • Received Date : 2022-11-07
  • Revised Date : 2022-11-15
  • Accepted Date : 2022-11-16
  • DOI :https://doi.org/10.7745/KJSSF.2022.55.4.475
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