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2021 Vol.54, Issue 3 Preview Page

Original research article

Large Scale Cultivation of Bacillus velezensis CE 100 and Effect of Its Culture on Control of Citrus Melanose Caused by Diaporthe citri
Dong Ryul Lee1
,
Chaw Ei Htwe Maung2
,
Tae Gyu Choi1
,
Kil Yong Kim3*
1Student, Department of Agricultural and Biological Chemistry, Environmentally-Friendly Agricultural Research Centre, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Korea
2Postdoctoral Researcher, Department of Agricultural and Biological Chemistry, Environmentally-Friendly Agricultural Research Centre, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Korea
3Professor, Department of Agricultural and Biological Chemistry, Environmentally-Friendly Agricultural Research Centre, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Korea
*Corresponding Author
31 August 2021. pp. 297-310
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Abstract

Diaporthe citri is an important fungal pathogen which causes melanose in various citrus growing regions of the world. In our study, control of D. citri was performed by using effective biocontrol agent, Bacillus velezensis CE 100. Mycelial growth of D. citri was highly restricted by strain CE 100 in dual culture plates. Our study demonstrates large-scale inoculation of strain CE 100 in non-sterilized PB medium. In addition, bacterial culture filtrate (BCF) of strain CE 100 from the large-scale culture showed better growth inhibition of D. citri than those from small-scale culture in the laboratory. Moreover, microscopic observation revealed that hyphal morphology of the pathogen was significantly affected by BCF, probably because of various cell-wall degrading enzymes and metabolites produced by strain CE 100. In addition, 50% BCF of strain CE 100 inhibited D. citri conidial germination by over 80%. Treatments, applied by foliar spray with fungicide and strain CE 100 culture, significantly lowered melanose disease incidence more than did control. The results of this study indicate that large-scale culture of strain CE 100 was effective for control of D. citri by means of adequate active metabolites and cells. Therefore, we demonstrate biocontrol applicability of B. velezensis CE 100 on control of citrus melanose by developing a low-cost PB medium using affordable large-scale fermenter.

Effect of different treatments on melanose disease incidence on leaves of mandarin plants at 20 days after inoculation with D. citri. Statistical comparison between treatments was done by least significant difference (LSD) test at p < 0.05 level. Means with the same letter are not significantly different from each other. *Con- Control, CE 100- Treatment with B. velezensis CE 100 culture, F- Treatment with fungicide
Keywords
Bacillus velezensis CE 100
Diaporthe citri
Large-scale cultivation
Melanose
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Information
  • Publisher :Korean Society of Soil Science and Fertilizer
  • Publisher(Ko) :한국토양비료학회
  • Journal Title :Korean Journal of Soil Science and Fertilizer
  • Journal Title(Ko) :한국토양비료학회 학회지
  • Volume : 54
  • No :3
  • Pages :297-310
  • Received Date : 2021-07-05
  • Revised Date : 2021-08-03
  • Accepted Date : 2021-08-11
  • DOI :https://doi.org/10.7745/KJSSF.2021.54.3.297
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