Isolation and characterization of plant growth-promoting rhizobacteria from soil

Soyoun Lee; Hyeonji Kim; Sungwoo Kim; Kyungjin Kwak; Sugju Ko

doi:10.7745/KJSSF.2025.58.1.027

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2025 Vol.58, Issue 1 Preview Page Next Page

Original research article

Isolation and characterization of plant growth-promoting rhizobacteria from soil

28 February 2025. pp. 27-37

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Abstract

To isolate and select useful microbial resources for agricultural environments, 26 microbial species with diverse colors and morphologies were isolated from soil. Antifungal tests against three plant pathogens revealed that seven strains, including CH22-3 (identified as Burkholderia ambifaria) and AB22-1 (Bacillus amyloliquefaciens), exhibited antifungal activity, with CH22-3 and AB22-1 showing the strongest effects. Evaluation of plant growth-promoting traits such as nitrogen fixation, phosphate solubilization, IAA, and siderophore production showed that all seven strains demonstrated nitrogen fixation, with CH22-3 achieving the highest phosphate solubilization (135.6%), and AB22-1 exhibiting the highest levels of IAA production (8.0 µg ml^-1) and siderophore production (5.7 mm halo zone). Lettuce treated with AB22-1 at concentrations of 1×10⁵ to 1×10⁷ cfu/ml showed increases in shoot and root biomass by 26 - 31% and 18 - 20%, respectively, compared to the control group, indicating AB22-1 strain potential as a biofertilizer. These results underscore the need for further comparative studies with chemical fertilizers.

Growth-promoting effect of Bacillus amyloliquefaciens AB22-1 isolated from soil on lettuce.

Treatment			Leaf length (cm)	Leaf width (cm)	Number of leaves (per plant)	Fresh weight (per 10 plant)		Chlorophll (SPAD)
Treatment			Leaf length (cm)	Leaf width (cm)	Number of leaves (per plant)	Shoot	Root	Chlorophll (SPAD)
AB22-1 culture	10⁵	Seedling	21.8 bcd	17.0 bc	12.8 ab	130.3 c	20.0 a	32.0 a
		Transplanting	22.4 abcd	18.0 ab	12.3 ab	146.8 abc	20.0 a	31.5 a
		Post-transplanting	23.3 ab	18.4 ab	13.5 ab	162.0 a	20.9 a	31.1 a
	10⁶	Seedling	21.9 bcd	18.1 ab	12.7 ab	137.3 bc	20.1 a	33.2 a
		Transplanting	22.1 abcd	17.9 ab	13.7 a	147.5 abc	21.4 a	30.6 a
		Post-transplanting	21.0 d	17.9 ab	12.5 ab	128.6 c	20.1 a	33.6 a
	10⁷	Seedling	23.6 ab	18.0 ab	13.8 a	168.4 a	20.5 a	31.7 a
		Transplanting	23.8 a	18.7 a	14.0 a	159.0 ab	20.0 a	30.6 a
		Post-transplanting	23.3 ab	17.3 abc	13.0 ab	146.3 abc	18.4 a	30.8 a
Media (LB broth)			22.9 abc	17.8 ab	11.5 b	128.6 c	18.2 a	28.8 a
control			21.3 cd	16.0 c	12.0 ab	128.5 c	17.4 a	29.8 a

Keywords

Antifungal activity

PGP activity

PGPR

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Information

Publisher :Korean Society of Soil Science and Fertilizer
Publisher(Ko) :한국토양비료학회
Journal Title :Korean Journal of Soil Science and Fertilizer
Journal Title(Ko) :한국토양비료학회 학회지
Volume : 58
No :1
Pages :27-37
Received Date : 2024-11-04
Revised Date : 2024-12-16
Accepted Date : 2024-12-26
DOI :https://doi.org/10.7745/KJSSF.2025.58.1.027

Korean Journal of Soil Science and Fertilizer ISSN:0367-6315(Print) 2288-2162(Online) 한국토양비료학회 학회지

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