All Issue

2017 Vol.50, Issue 6 Preview Page
Paenibacillus polymyxa and Burkholderia cepacia Antagonize Ginseng Root Rot Pathogens
Young Don Lee1
,
Khalid Abdullah Hussein1,2
,
Jin Ho Joo1*
,
,
1Department of Biological Environment, Kangwon National University
,2Botany and Microbiology Department, Faculty of Science, Assiut University,
*Corresponding Author. 
December 2017. pp. 598-605
PDF XML Citation
Abstract

To isolate rhizobacteria exhibiting antifungal activities for for five pathogenic fungi (Sclerotinia sclerotiorum, Fusarium solani, Collectotricum gloeosporides, Fusarium oxysporum, and Botrytis cinerea) which cause damage to Ginseng root in Ginseng grown fields, four soils were collected from Cheorlwon gun, in Korea. From 4 soils, a total of 160 bacterial strains were isolated by dilution plate method. Among 160 strains, 32 strains showed antifungal activities for one or more pathogens. From 32 strains, three strains exhibited antifungal activities for all pathogens. These are two Burkholderia cepacia (ATCC 25416 and ET 13) and one Paenibacillus polymyxa (ATCC 842). These potent antifungal strains showed high identities (99% using 16S-rRNA sequencing).

Identification of 3 strains showing antifungal activities for five pathogens

Keywords
Pathogenic fungi
Ginseng
Antifungal bacteria
Sclerotinia sclerotiorum

References
1
Chand, G. and K. Santosh. 2015. Crop diseases and their management. Taylor & Francis. 302-305
2
Chung, Y.K. and M.S. Chang. 2007. Conception and argumentation of sustainable development. Kor. East-West. Eco. Assoc. 19(1):143-164
3
El-Banna. N. and G. Winkelmann. 1998. Pyrrolnitrin from burkholderia cepacia: antibiotic activity againist fungi and novel activities againist streptomycetes. J. Appl. Microbiol. 85(1):69-78
4
Hartley, C.P. 1912. Use of soil fungicides to prevent damping-off of coniferous seedlings. Soc. Amer. Foresters. Proc. 7:96-99.
5
KEFAMA. 2012. Standard guidelines of environment-friendly organic materials. Korea Eco-Friendly Agro- Materials Association.
6
KFDA. 2005. Survey of consumer awareness about organic food and organic food products. Korea Food and Drug Administration.
7
Kim, B.Y., J.H. Ahn, H.Y. Weon, J.K. Song, S.I. Kim, and W.G. Kim. 2012. Isolation and characterization of bacillus species possessing antifungal activity against ginseng root rot pathogens. Korean Soc. Pesticide Sci. 16(4): 357-363.
8
KMIFAFF. 2009. Environmentaly – friendly agriculture promotion act. Korea, Ministry for Food, Agriculture, Forestry and Fisheries. No 9623.
9
Nam, S.W. 2006. Isolation of antagonistic microorganisms and biological control against large patch caused by rhizoctonia solani AG2-2 IV in zoysiagrass. Master’s degree. Thesis, Kunkuk University. Seoul. Korea.
10
NIAS. 2000. Soil and plants analysis method. National Institute of Agricultural Sciences. Korea.
11
NIAS. 2010. Soil chemical analysis. National Institute of Agricultural Sciences. Korea.
12
Park, S.J. 2013. Controlling Effect of Agricultural Organic Materials on phytophthora blight and Anthracnose in red papper. Res. Plant Dis. 18(1):1-9.
13
Rios, J.L., M.C. Recio, and A. Villar. 1988. Screening methods for natural products with antmicirobial activity: A review of the literature. J. Ethno pharmacol. 23:127-149.
14
Shaheen, M., J. Li, A.C. Ross, J.C. Vederas, and S.E. Jensen. 2011. Paenibacillus polymyxa PKB1 produces variants of polymyxin β-Type antibiotics. Chem. Biol. 18(12):1640-1648
15
Yang, J.E., J.B. Jung, G.W. Kim, G.Y. Kim, S.J. Kim, S.M. Kim, J.U. Kim, J.S. Seo, B.G. Son, J.H. Sim, Y. Ahn, J.H. Ahn, G.C. Um, Y.S. Ok, S.T. Yun, G.S. Lee, D.B. Lee, Y.D. Lee, J.T. Lee, D.Y. Jung, Y.S. Jung, J.B. Jung, J.S. Jo, S.H. Choi, J.S. Heo, H.N. Hyun, S.Y. Hong. 2008. Ag-environmental Science. 2:304-305. CIR.
16
Yang, S.J. 2011. The selection and characteristics of antagonistic microorganisms for major pathogens on paprika. Master’s degree Thesis. Chonbuk National University. Jeonju. Korea.
Information
  • Publisher :Korean Society of Soil Science and Fertilizer
  • Publisher(Ko) :한국토양비료학회
  • Journal Title :Korean Journal of Soil Science and Fertilizer
  • Journal Title(Ko) :한국토양비료학회 학회지
  • Volume : 50
  • No :6
  • Pages :598-605
  • Received Date : 2017-10-16
  • Revised Date : 2017-11-15
  • Accepted Date : 2017-11-16
  • DOI :https://doi.org/10.7745/KJSSF.2017.50.6.598
Journal Informaiton Korean Journal of Soil Science and Fertilizer Korean Journal of Soil Science and Fertilizer
KJSSF Online Submission
  • NRF
  • KOFST
  • crossref crossmark
  • crossref cited-by
  • crosscheck
  • orcid
  • open access
  • ccl
Journal Informaiton Journal Informaiton - close