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2017 Vol.50, Issue 2 Preview Page
Analysis of Soil Total Nitrogen and Inorganic Nitrogen Content for Evaluating Nitrogen Dynamics
Seul-Bi Lee1*
,
Jwa-Kyung Sung1
,
Ye-Jin Lee1
,
Jung-Eun Lim1
,
Yo-Sung Song1
,
Deog-Bae Lee1
,
Suk-Young Hong1
,
,
,
,
,
,
1Division of Soil & Fertilizer, National Institute of Agricultural Science
*Corresponding Author. 
April 2017. pp. 100-105
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Abstract

Various methods for assessing soil total nitrogen (TN) and inorganic N content have been developed to manage nutrient and to understand N cycle in soil. This paper address the technical procedures in arable soil samples to conduct soil sampling, sample preparation, and measuring total N and inorganic N. Among various methods for measuring soil total nitrogen contents, Kjeldahl distillation and Indophenol blue method have widely used due to reliability and economic advances. Also, two methods can analyze more samples at the same time compared with other nitrogen measuring methods. For evaluating inorganic N content, mainly in forms of nitrate-N (NO3--N) and ammonium-N (NH4+-N), extraction with a single reagent such as 2M KCl has been employed, followed by Kjeldahl distillation or indophenol blue methods.

Analysis procedure of total nitrogen and inorganic nitrogen in soil samples.

Keywords
Ammonium nitrogen
Indophenol blue
Kjeldahl method
Nitrate nitrogen
Total nitrogen

References
1
Biggar, J.W. 1978. Spatial variability of nitrogen in soils. In D. R. Nielsen and J. G. MacDonald (ed.) Nitrogen in the Environment. 1:201-211. Acad. Press, New York.
2
Breimer, T. and J.H.G. Slangen. 1981. Pretreatment of soil samples before NO3-N analysis. Neth. J. Agric. Sci. 29:15-22.
3
Bremner, J.M. and D.R. Keeney. 1965. Steam distillation methods for determination of ammonium, nitrate and nitrite. Anal. Chim. Acta. 32:485-495.
4
Fageria, N.K.  and V.C. Baligar. 2005. Enhancing nitrogen use efficiency in crop plants. Adv. Agron. 88:97-185.
5
Henry, H.A.L. and R.L. Jefferies. 2003. Plant amino acid uptake, soluble N turnover and microbial N capture in soils of a grazed arcticsalt marsh. J. Ecol. 91:627-636.
6
Keeney, D.R. and D.W. Nelson. 1982. Nitrogen-inorganic forms. p. 643-698. In A.L. Page et al. (ed.) Methods of Soil Analysis. Part 2.2nd ed. Agron.Monogr. 9. ASA and SSSA. Madison, WI.
7
Keeney, D.R. and J.M. Bremner. 1967. Use of the Coleman Model 29A Analyzer for total nitrogen analysis of soils. Soil Sci. 104:358-363.
8
Li, S.X., Z.H. Wang,  and B.A. Stewart. 2013. Responses of crop plants to ammonium and nitrate N. Adv. Agron. 118:205-397.
9
Liu, J.X., S.J. Peng, B. Faivre-vuillin, Z.H. Xu, D.Q. Zhang, and G.Y. Zhou. 2008. Erigeron annuus (L.) Pers., as a green manure for ameliorating soil exposed to acid rain in Southern China. J. Soil Sediment. 8:452-460.
10
National Institute of Agricultural Science and Technology (NIAST). 2000. Method of Soil and Plant Analysis. National Institute of Agricultural Science and Technology, RDA. Korea.
11
Nordin, A.P., I.K. Schmidt, and G.R. Shaver. 2004. Nitrogen uptake by arctic soil microbes and plants in relation to soil nitrogen supply. Ecol. 85:955-962.
12
Pansu, M. and J. Gautheyrou. 2007. Handbook of Soil Analysis: Mineralogical, Organic and Inorganic Methods. Springer Science & Business Media.
13
Rabalais, N.N., R. E. Turner, and W.I. Wiseman Jr. 2002. Gulf of Mexico hypoxia, aka “The dead zone”. Annu. Rev. Ecol. Evol. S. 33(1):235-263.
14
RDA. 2012. Method of Soil Chemical Analysis. Standard methods for Agricultural Science and Technology Experiment. Rural Development of Administration. Korea.
15
Schimel, J.P. and J. Bennett. 2004. Nitrogen mineralization: challenges of a changing paradigm. Ecol. 85(3): 591-602.
16
Schulten, H.R. and M. Schnitzer. 1998. The chemistry of soil organic nitrogen: a review. Biol. Fertil. Soils 26:1-15.
17
Scolow, R.W. 1999. Nitrogen management and the future of food: Lessons from management of energy and carbon. Proc. Natl.Acad.Sci. USA. 96:6001-6008.
18
Sparks, D.L. 1996. Methods of Soil Analysis, part 3 (SSSA Book Series No.5). Madison, Wisc.: SSSA and ASA.
19
Stewart, B.A., L.K. Poter, and F.E. Clark. 1963. The reliability of a micro-Dumas procedure for determining total nitrogen in soil. Soil Sci. Soc. Am. Proc. 27: 377-380.
20
Young, J.L. 1962. Inorganic soil nitrogen and carbon:nitrogen ratios of some Pacific Northwest soils. Soil Sci. 93: 397-404.
Information
  • Publisher :Korean Society of Soil Science and Fertilizer
  • Publisher(Ko) :한국토양비료학회
  • Journal Title :Korean Journal of Soil Science and Fertilizer
  • Journal Title(Ko) :한국토양비료학회 학회지
  • Volume : 50
  • No :2
  • Pages :100-105
  • Received Date : 2017-02-08
  • Revised Date : 2017-04-26
  • Accepted Date : 2017-04-26
  • DOI :https://doi.org/10.7745/KJSSF.2017.50.2.100
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