All Issue

2024 Vol.57, Issue 2

Original research article

Nitrogen fertilization-affected metabolite profiling in sorghum-sudangrass hybrid plant
Bomin Seo1
,
Jwakyung Sung2*
1Researcher, Chungbuk Agricultural Research and Extension Service, Cheongju 28130, Korea
2Associate Professor, Department of Crop Science, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 28644, Korea
*Corresponding Author
31 May 2024. pp. 79-87
PDF XML Citation
Abstract

Nitrogen (N), an essential macronutrient for crop growth and development, is widely used as a fertilizer. Balanced nitrogen is believed to be responsible for production and allocation of metabolites. Therefore, this study was aimed to understand the impact of different levels of nitrogen fertilization on the dynamics of primary metabolites. Sorghum-sudangrass hybrid was experimented with different nitrogen levels (N0, N100 and N200), and sampled at heading and harvesting stages. The results showed that metabolic profiling of a sorghum-sudangrass hybrid revealed distinct responses to nitrogen fertilization in leaf and stem tissues. Higher nitrogen levels resulted in the up-regulation of sugars and amino acids while down-regulating organic acids. Amino acids, especially glutamine and asparagine, were more abundant in stem with higher nitrogen at harvesting. Further analysis confirmed an impact of nitrogen fertilization on monosaccharides and amino acids, with amino acids exhibiting higher stem abundance. Principal component analysis highlighted strong correlations between selected metabolites and nitrogen levels, particularly at higher concentrations. In summary, the study demonstrates that nitrogen fertilization is significantly responsible for an impact on the metabolic profile of the sorghum-sudangrass hybrid, particularly in sugars and amino acids. These findings contribute valuable insights into the plant’s response to varying nitrogen levels, emphasizing the importance of nitrogen fertilization in shaping metabolic dynamics.

Correlation between selected metabolites in the leaves and stems.
Keywords
Nitrogen fertilization
Primary metabolites
Sorghum-sudangrass hybrid
References
1

Afzal M, Ahmad A, Zamir S. 2013. Performance of multicut forage sorghum under various sowing methods and nitrogen application rates. J. Anim. Plant Sci. 23:232-239.

2

Beyaert R, Roy R. 2005. Influence of nitrogen fertilization on multi-cut forage sorghum-sudangrass yield and nitrogen use. Agron. J. 97:1493-1501. https://doi.org/10.2134/agronj2005.0079

10.2134/agronj2005.0079
3

Choi N, Kim G, Park W, Jeong Y, Kim YH, Na CI. 2022. Additional N application and ecotype affect yield and quality of ratoon harvested sorghum x sudangrass hybrid for temperate regions. Biomass and Bioenergy 160:106423. https://doi.org/10.1016/j.biombioe.2022.106423

10.1016/j.biombioe.2022.106423
4

Choi GJ, Lee SH, Lee KW, Chung HJ, Hwang TY, Kim KY. 2017. Comparison of growth characteristics, quantity, and feed value between Sudanese-Sudangrass hybrid and Susu-Sudangrass hybrid. J. Korean Soc. Grassl. Forage Sci. 37:92-99. https://doi.org/10.5333/KGFS.2017.37.1.92

10.5333/KGFS.2017.37.1.92
5

Chung RS, Chen CC, Ng LT. 2010. Nitrogen fertilization a_ects the growth performance, betaine and polysaccharide concentrations of Lycium barbarum. Ind. Crop Prod. 32:650-655. https://doi.org/10.1016/j.indcrop.2010.07.021

10.1016/j.indcrop.2010.07.021
6

Frink CR, Waggoner PE, Ausubel JH. 1999. Nitrogen fertilizer: Retrospect and prospect. Proc. Natl. Acad. Sci. 96:1175-1180. https://doi.org/10.1073/pnas.96.4.1175

10.1073/pnas.96.4.11759989997PMC33552
7

Hildebrandt TM, Nunes Nesi A, Araujo WL, Braun HP. 2015. Amino acid catabolism in plants. Mol. Plant 8:1563-1579. http://dx.doi.org/10.1016/j.molp.2015.09.005

10.1016/j.molp.2015.09.00526384576
8

Kim YB, Park SY, Park CH, Park WT, Kim SJ, Ha SH, Arasu MV, Al-Dhabi NA, Kim JK, Park SU. 2016. Metabolomics of differently colored Gladiolus cultivars. Appl. Biol. Chem. 59:597-607. http://dx.doi.org/10.1007/s13765-016-0197-0

10.1007/s13765-016-0197-0
9

Madore MA. 2001. Biosynthesis and degradation of galactosyloligosaccharides. pp. 1662-1690. In Fraser-Reid BO et al. (Eds.) Glycoscience: Chemistry and chemical biology I-III. Springer-Verlag, Berlin, Heidelberg. https://doi.10.1007/978-3-642-56874-9

10.1007/978-3-642-56874-9_39
10

MAFRA. 2022. Annual statistics of crop production. Ministry of Agriculture, Food and Rural Affairs, Sejong, Korea.

11

McKinley BA, Thakran M, Zemelis-Durfee S, Huang X, Brandizzi F, Rooney WL, Mansfield SD, Mullet JE. 2022. Transcriptional regulation of the raffinose family oligosaccharides pathway in Sorghum bicolor reveals potential roles in leaf sucrose transport and stem sucrose accumulation. Front. Plant Sci. 13:1062264. https://doi.10.3389/fpls.2022.1062264.

10.3389/fpls.2022.106226436570942PMC9785717
12

NAAS (National Academy of Agricultural Science). 2010. Methods of soil analysis. RDA, Suwon, Korea.

13

Pedersen JF. 1996. Annual forages: New approaches for C-4 forages. pp. 246-251. In Janick J (Ed.), Progress in new crops, ASHS Press, Alexandria, VA, USA.

14

Reddy B, Ramesh S, Reddy P, Ramaiah B, Salimath M, Kachapur R. 2005. Sweet sorghum - A potential alternate raw material for bio-ethanol and bio-energy. Int. Sorghum Millets Newsl. 46:79-86.

15

Rufty TW, Huber SC, Volk RJ. 1988. Alterations in leaf carbohydrate metabolism in response to nitrogen stress. Plant Physiol. 88:725-730. https://doi.org/10.1104/pp.88.3.725

10.1104/pp.88.3.72516666374PMC1055651
16

Sebastia CH, Marsolais F, Saravitz C, Israel D, Dewey RE, Huber SC. 2005. Free amino acid profiles suggest a possible role for asparagine in the control of storage-product accumulation in developing seeds of low- and high-protein soybean lines. J. Exp. Bot. 56:1951-1963. https://doi.org/10.1093/jxb/eri191

10.1093/jxb/eri19115911557
17

Shi Z, Wei F, Wan R, Li Y, Wang Y, An W, Qin K, Dai G, Cao Y, Feng J. 2019. Impact of nitrogen fertilizer levels on metabolite profiling of the Lycium barbarum L. fruit. Molecules 24:3879. https://doi.org/10.3390/molecules24213879

10.3390/molecules2421387931661883PMC6864581
18

Tilsner J, Kassner N, Struck C, Lohaus G. 2005. Amino acid contents and transport in oilseed rape (Brassica napus L.) under different nitrogen conditions. Planta 221:328-338. https://doi.org/10.1007/s00425-004-1446-8

10.1007/s00425-004-1446-815599760
19

Urbanczyk-Wochniak E, Fernie AR. 2005. Metabolic profiling reveals altered nitrogen nutrient regimes have diverse effects on the metabolism of hydroponically-grown tomato (Solanum lycopersicum) plants. J. Exp. Bot. 56:309-321. https://doi.org/10.1093/jxb/eri059

10.1093/jxb/eri05915596475
20

Uzun F, Ugur S, Sulak M. 2009. Yield, nutritional and chemical properties of some sorghum x sudangrass hybrids (Sorghum bicolor (L.) Moench x Sorghum sudanense Stapf.). J. Anim. Vet. Adv. 8:1602-1608.

21

Yoon SH, Kim JG, Jeong ES, Sung SH. 2007. The study on double cropping system for organic forage production in middle part of Korea. J. Korean Soc. Grassl. Forage Sci. 27:275-280. https://doi.org/10.5333/KGFS.2007.27.4.275

10.5333/KGFS.2007.27.4.275
22

Zhen S, Zhou J, Deng X, Zhu G, Cao H, Wang Z, Yan Y. 2016. Metabolite profiling of the response to high-nitrogen fertilizer during grain development of bread wheat (Triticum aestivum L.). J. Cereal Sci. 69:85-94. https://doi.org/10.1016/j.jcs.2016.02.014

10.1016/j.jcs.2016.02.014
Information
  • Publisher :Korean Society of Soil Science and Fertilizer
  • Publisher(Ko) :한국토양비료학회
  • Journal Title :Korean Journal of Soil Science and Fertilizer
  • Journal Title(Ko) :한국토양비료학회 학회지
  • Volume : 57
  • No :2
  • Pages :79-87
  • Received Date : 2024-01-26
  • Revised Date : 2024-05-10
  • Accepted Date : 2024-05-22
  • DOI :https://doi.org/10.7745/KJSSF.2024.57.2.079
Journal Informaiton Korean Journal of Soil Science and Fertilizer Korean Journal of Soil Science and Fertilizer
KJSSF Online Submission
  • NRF
  • KOFST
  • crossref crossmark
  • crossref cited-by
  • crosscheck
  • orcid
  • open access
  • ccl
Journal Informaiton Journal Informaiton - close