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2021 Vol.54, Issue 4 Preview Page

Short communication

A Simple Method to Determine Soil Microbial Biomass Carbon by Facilitating Gaseous Diffusion of Chloroform into Pores
Jeong Su Lee1
,
Yehun Lee1
,
Eun-jin Lee2
,
Khok Pros3
,
Kyoungyoung Kim1
,
Gwang Hyun Han4*
1Master Student, Department of Environmental and Biological Chemistry, Chungbuk National University, Cheongju 28644, Korea
2Research Scientist, Department of Soil and Fertilizer, National Institute of Agricultural Science, RDA, Wanju 55365, Korea
3Doctoral Student, Department of Environmental and Biological Chemistry, Chungbuk National University, Cheongju 28644, Korea
4Professor, Department of Environmental and Biological Chemistry, Chungbuk National University, Cheongju 28644, Korea
*Corresponding Author
30 November 2021. pp. 660-666
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Abstract

Chloroform fumigation and extraction (CFE) has been a widely accepted method for quantifying overall size of soil microbial community, which is a key indicator to assess the quality and health of a given soil. However, the standard procedure of CFE requires careful operations to generate enough CHCl3 vapors and flush them into soil pores. We suggest a new simple alternative for facilitating gaseous diffusion of CHCl3 molecules under ambient air pressure conditions. The microbial C values by the new approach, examined for several farmland, forest, and grassland soils, coincided well with those obtained by the standard CFE. The cell lysis and extraction coefficient was also close to that of standard CFE and much higher than that evaluated for a liquid-based CHCl3 cell lysis.

The microbial C values by the new approach (GD) coincided well with those obtained by the standard CFE, compared with those measured by a liquid-based CHCl3 cell lysis (LC).
Keywords
Chloroform fumigation and extraction
Extraction coefficient
Soil health
Soil quality
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Information
  • Publisher :Korean Society of Soil Science and Fertilizer
  • Publisher(Ko) :한국토양비료학회
  • Journal Title :Korean Journal of Soil Science and Fertilizer
  • Journal Title(Ko) :한국토양비료학회 학회지
  • Volume : 54
  • No :4
  • Pages :660-666
  • Received Date : 2021-11-02
  • Revised Date : 2021-11-08
  • Accepted Date : 2021-11-08
  • DOI :https://doi.org/10.7745/KJSSF.2021.54.4.660
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