All Issue

2019 Vol.52, Issue 4 Preview Page

Original research article

Isolation and Characterization of Phosphate Solubilizing Pseudomonas Species and Their Effect on Plant Growth Promotion
Gyeong Seo Jo1*
,
Sang Yoon Kim1
,
Ju Sik Cho1
,
Yong Hwa Cheong1
1Department of Bio-Environmental Science, Sunchon National University
* Corresponding Author
30 November 2019. pp. 438-447
PDF XML Citation
Abstract

Plant growth-promoting rhizobacteria (PGPR) affect plant growth via various direct or indirect mechanisms. These microorganisms are the potential tools for sustainable agriculture. In this study, eight different Pseudomonas strains (PM1 - PM8) isolated from rhizospheric soil were characterized plant growth-promoting characteristics, including indole-3-acetic acid (IAA) and Gibberellin (GA3) production, phosphate solubilization, 1- aminocyclopropane-1-carboxylic acid (ACC) deaminase activity and effects of direct inoculation on plant growth parameters (fresh weight, shoot length). All of these strains have their ability for solubilization of insoluble phosphate depending on pH decrease at the range around pH4 - 5 at 2 days after inoculation. PM1, PM4, PM5 and PM7 species was capable of IAA production and PM4, PM5, PM7 and PM8 species have high activity for production of gibberellin (GA3) hormone. For ACC deaminase activity, PM1, PM3 and PM8 show high activity. Furthermore, all these species have significantly promoted the growth of the lettuce seedling plants for fresh weight and shoot length enhancement, so that these Pseudomonas species could be used as potential bio-fertilizer agents.

Production of plant hormone IAA (A) and GA3 (B) from isolated Pseudomonas species.

Keywords
Plant growth promoting
Phosphate solubilizing bacteria
IAA and GA3 production
ACC deaminase activity
Pseudomonas species

References
1
Backer, R., J.S. Rokem, G. Ilangumaran, J. Lamont, D. Praslickova, E. Ricci, S. Subramanian, and D.L. Smith. 2018. Plant growth-promoting rhizobacteria: context, mechanisms of action, and roadmap to commercialization of bostimulants for sustainable agriculture. Front. Plant Sci. 9:1473.
10.3389/fpls.2018.0147330405652PMC6206271
2
Bal, H.B., S. Das, T.K. Dangar, and T.K. Adhya. 2013. ACC deaminase and IAA producing growth promoting bacteria from the rhizosphere soil of tropical rice plants. J. Basic Microbiol. 53:972-984.
10.1002/jobm.20120044523681643
3
Beattie, G.A. 2015. Microbiomes: curating communities from plants. Nature. 528:340-341.
10.1038/nature1631926633626
4
Bender, S.F., C. Wagg, and M.G. Van Der Heijden. 2016. An underground revolution: biodiversity and soil ecological engineering for agricultural sustainability. Trends Ecol. Evol. 31:440-452.
10.1016/j.tree.2016.02.01626993667
5
Bhattacharyya, P.N. and D.K. Jha. 2012. Plant growth-promoting rhizobacteria (PGPR): emergence in agriculture. World J. Microbiol. Biotechnol. 28:1327-1350.
10.1007/s11274-011-0979-922805914
6
Bloemberg, G.V. and B. Lugtenberg. 2001. Molecular basis of plant growth promotion and biocontrol by rhizobacteria. Curr. Opin. Plant Biol. 4:343-350.
10.1016/S1369-5266(00)00183-7
7
Castagno, L.N., M.J. Estrella, A.I. Sannazzaro, A.E. Grassano, and O.A. Ruiz. 2011. Phosphate-solubilization mechanism and in vitro plant growth promotion activity mediated by Pantoea eucalypti isolated from Lotus tenuis rhizosphere in the Salado River Basin (Argentina). J. Appl. Microbiol. 110:1151-1165.
10.1111/j.1365-2672.2011.04968.x21299771
8
Dey, R., K.K. Pal, D.M. Bhatt, and S.M. Chauhan. 2004. Growth promotion and yield enhancement of peanut (Arachishypogaea L.) by application of plant growth promoting rhizobacteria. Microbiol. Res. 159:371-394.
10.1016/j.micres.2004.08.00415646384
9
Ellis, R.J., T.M. Timms-Wilson, and M.J. Bailey. 2000. Identification of conserved traits in fluorescent pseudomonads with antifungal activity. Environ. Microbiol. 2:274-284.
10.1046/j.1462-2920.2000.00102.x11200428
10
Garrido-Sanz, D., J.P. Meier-Kolthoff, M. Göker, M. Martín, R. Rivilla, and M. Redondo-Nieto. 2016. Genomic and genetic diversity within the Pseudomonas fluorescens complex. PLoS ONE 11:e0150183.
10.1371/journal.pone.015018326915094PMC4767706
11
Glick, B.R. 1995. The enhancement of plant growth by free living bacteria. Can. J. Microbiol. 4:1109-1114.
10.1139/m95-015
12
Glick, B.R. 2014. Bacteria with ACC deaminase can promote plant growth and help to feed the world. Microbiol. Res. 169:30-39.
10.1016/j.micres.2013.09.00924095256
13
Gordon, S.A. and R.P. Weber. 1951. Colorimetric estimation of indole acetic acid. Plant Physiol. 2:192-195.
10.1104/pp.26.1.19216654351PMC437633
14
Gupta, G., S.S. Parihar, N.K. Ahirwar, S.K. Snehi, and V. Singh. 2015. Plant growth promoting rhizobacteria (PGPR): current and future prospects for development of sustainable agriculture. J. Microb. Biochem. Technol. 7:96-102.
15
Gupta, S. and S. Pandey. 2019. ACC deaminase producing bacteria with multifarious plant growth promoting traits alleviates salinity stress in french bean (Phaseolus vulgaris) plants. Front. Microbiol. 10:1506.
10.3389/fmicb.2019.0150631338077PMC6629829
16
Heydarian, Z., M. Yu, M. Gruber, B.R. Glick, R. Zhou, and D.D. Hegedus. 2016. Inoculation of soil with plant growth promoting bacteria producing 1-aminocyclopropane-1-carboxylate deaminase or expression of the corresponding acds gene in transgenic plants increases salinity tolerance in camelina sativa. Front. Microbiol. 7:1966.
10.3389/fmicb.2016.0196628018305PMC5159422
17
Holbrook, A., W. Edge, and F. Bailey. 1961. Spectrophotometric method for determination of gibberellic acid. Adv. Chem. Ser. 28:159-167.
10.1021/ba-1961-0028.ch018
18
Illmer, P.A. and F. Schinner. 1992. Solubilization of inorganic phosphates by microorganisms isolated from forest soil. Soil Biol. Biochem. 24:389-395.
10.1016/0038-0717(92)90199-8
19
Islam, S., A.M. Akanda, A. Prova, M.T. Islam, and M.M. Hossain. 2016. Isolation and identification of plant growth promoting rhizobacteria from cucumber rhizosphere and their effect on plant growth promotion and disease suppression. Front. Microbiol. 6:1360.
10.3389/fmicb.2015.0136026869996PMC4735380
20
Jeon, J.S., S.S. Lee, H.Y. Kim, T.S. Ahn, and H.G. Song. 2003. Plant growth promotion in soil by some inoculated microorganisms. J. Microbiol. 41:271-276.
21
Khan, A.A., G. Jilani, M.S. Akhtar, S.M.S. Naqvi, and M. Rasheed. 2009. Phosphorus solubilizing bacteria: occurrence, mechanisms and their role in crop production. J. Agric. Biol. Sci. 1:48-58.
22
Kumar, S., K. Tamura, I.B. Jakobsen, and M. Nei. 2001. MEGA2: molecular evolutionary genetics analysis software. Bioinformatics. 17:1244-1245.
10.1093/bioinformatics/17.12.124411751241
23
Leveau, J.H.J. and S.E. Lindow. 2005. Utilization of the plant hormone indole-3-acetic acid for growth by Pseudomonas putida strain 1290. Appl. Environ. Microbiol. 71:2365-2371.
10.1128/AEM.71.5.2365-2371.200515870323PMC1087548
24
Loper, J.E., K.A. Hassan, D.V. Mavrodi, E.W. Davis, C.K. Lim, B.T. Shaffer, L.D.H. Elbourne, V.O. Stockwell, S.L. Hartney, K. Breakwell, M.D. Henkels, S.G. Tetu, L.I. Rangel, T.A. Kidarsa, N.L. Wilson, J.E. van de Mortel, C. Song, R. Blumhagen, D. Radune, J.B. Hostetler, L.M. Brinkac, A.S. Durkin, D.A. Kluepfel, W.P. Wechter, A.J. Anderson, Y.C. Kim, L.S. Pierson, E.A. Pierson, S.E. Lindow, D.Y. Kobayashi, J.M. Raaijmakers, D.M. Weller, L.S. Thomashow, A.E. Allen, and I.T. Paulsen. 2012. Comparative genomics of plant-associated Pseudomonas spp.: insights into diversity and inheritance of traits involved in multitrophic interactions. PLoS Genet. 8:e1002784.
10.1371/journal.pgen.100278422792073PMC3390384
25
Lugtenberg, B. and F. Kamilova. 2009. Plant-growth-promoting rhizobacteria. Annu. Rev. Microbiol. 63:541-556.
10.1146/annurev.micro.62.081307.16291819575558
26
Morgan, P.W. and M.C. Drew. 1997. Ethylene and plant responses to stress. Physiol. Plant. 100:620-630.
10.1111/j.1399-3054.1997.tb03068.x
27
Murphy, J. and J.P. Riley. 1962. A modified single solution method for the determination of phosphate in natural waters. Anal. Chim. Acta. 27:265-270.
10.1016/S0003-2670(00)88444-5
28
Omer, Z.S., R. Tombolini, A. Broberg, and B. Gerhardson. 2004. Indole-3-acetic acid production by pink-pigmented facultative methylotrophic bacteria. Plant Growth Regul. 43:93-96.
10.1023/B:GROW.0000038360.09079.ad
29
Penrose, F.D.M. and B.R. Glick. 2003. Methods for isolating and characterizing ACC deaminase-containing plant growth promoting rhizobacteria. Physiol. Plant. 118:10-15.
10.1034/j.1399-3054.2003.00086.x12702008
30
Pii, Y., T. Mimmo, N. Tomasi, R. Terzano, S. Cesco, and C. Crecchio. 2015. Microbial interactions in the rhizosphere: beneficial influences of plant growth-promoting rhizobacteria on nutrient acquisition process. Biol. Fertil. Soils. 51:403-415.
10.1007/s00374-015-0996-1
31
Pikovskaya, R.I. 1948. Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Microbiologiya. 17:362-370.
32
Rengel, Z. and P. Marschner. 2005. Nutrient availability and management in the rhizosphere: exploiting genotypic differences. New Phytol. 168:305-312.
10.1111/j.1469-8137.2005.01558.x16219070
33
Rodriguez, H. and R. Fraga. 1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnol. Adv. 17:319-339.
10.1016/S0734-9750(99)00014-2
34
Saber, K., L.D. Nahla, and A. Chedly. 2005. Effect of P on nodule formation and N fixation in bean. Agron. Sustain. Dev. 25:389-393.
10.1051/agro:2005034
35
Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenic trees. Mol. Biol. Evol. 4:406-425.
36
Santoro, M.V., L.R. Cappellari, W. Giordano, and E. Banchio. 2015. Plant growth-promoting effects of native Pseudomonas strains on Mentha piperita (peppermint): an in vitro study. Plant Biol. (Stuttg). 17:1218-1226.
10.1111/plb.1235126012535
37
SAS. 1999. SAS/STAT User's Guide Version 8. SAS. Cary. NC.
38
Sharma, S.B., R.Z. Sayyed, M.H. Trivedi, and T.A. Gobi. 2013. Phosphate solubilizing microbe: sustainable approach for managing phosphorus deficiency in agricultural soils. SpringerPlus. 2:587.
10.1186/2193-1801-2-58725674415PMC4320215
39
Steel, R.G.D. and J.H. Torrie. 1980. Principles and procedures of statistics 2nd Eds. New York, NY: McGraw Hill Book Co. Inc.
40
Thompson, J.D., T.J. Gibson, F. Plewniak, F. Jeanmougin, and D.G. Higgins. 1997. The clustal x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25:4876-4882.
10.1093/nar/25.24.48769396791PMC147148
41
Vessey, J.K. 2003. Plant growth promoting rhizobacteria as biofertilizers. Plant Soil. 255:571-586.
10.1023/A:1026037216893
Information
  • Publisher :Korean Society of Soil Science and Fertilizer
  • Publisher(Ko) :한국토양비료학회
  • Journal Title :Korean Journal of Soil Science and Fertilizer
  • Journal Title(Ko) :한국토양비료학회 학회지
  • Volume : 52
  • No :4
  • Pages :438-447
  • Received Date : 2019-09-19
  • Revised Date : 2019-11-04
  • Accepted Date : 2019-11-05
  • DOI :https://doi.org/10.7745/KJSSF.2019.52.4.438
Journal Informaiton Korean Journal of Soil Science and Fertilizer Korean Journal of Soil Science and Fertilizer
KJSSF Online Submission
  • NRF
  • KOFST
  • crossref crossmark
  • crossref cited-by
  • crosscheck
  • orcid
  • open access
  • ccl
Journal Informaiton Journal Informaiton - close