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2020 Vol.53, Issue 2 Preview Page

Original research article

Effect of Bacillus CC112 Inoculation on Fungal Pathogens and Soil Microbial Community in a Ginseng-Cultivated Soil
Sang Yoon Kim17
,
Ju-Hee An2
,
Kyung Hoon Park3
,
Sang Yeob Lee4
,
Hang-Yeon Weon5
,
Mee-Kyung Sang5
,
Ju-Hee Lee6
,
Jaekyeong Song4*
1Professor, Department of Bio-Environmental Sciences, Sunchon National University, Suncheon 57922, Korea
2Assistant Researcher, Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea
3Researcher, Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong 27709, Korea
4Senior Researcher, Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea
5Researcher, Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea
6Graduate Student, Department of Bio-Environmental Sciences, Sunchon National University, Suncheon 57922, Korea
7Postdoctoral Researcher, Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea
* Corresponding Author
31 May 2020. pp. 128-139
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Abstract

Inoculation of beneficial bacteria such as Bacillus velezensis strains has been widely used to stimulate plant growth and suppress the activity of fungal pathogens in agricultural fields including ginseng (Panax ginseng). However, there is little information on changing microbial abundances and community structure. In this study, we investigated the inoculum populations and pathogenic abundances (Rhizoctonia solani and Pythium ultimum) by quantitative real time PCR (qPCR) and the shift of bacterial communities by Illumina Miseq based on 16s rRNA gene in the soil cultivated with ginseng under the two different inoculation methods (drenching and seed soaking). The population of inoculated microbe (Bacillus velezensis CC112) has maintained its relevant proportion for 4 to 9 days during the experiment in a ginseng-cultivating soil. The abundance of pathogenic fungi was effectively suppressed by the application of the Bacillus strain against Rhizoctonia solani (39%) and Pythium ultimum (7%), respectively as compared to the control (no application) without altering significant bacterial community structure in a ginseng soil. The inoculation of B. velezensis CC112 maintained its abundance almost until two weeks after the inoculation without significantly altering bacterial community structures in a ginseng soil. Conclusively, the inoculation of beneficial microbes is capable of potentially suppressing plant pathogenic fungi and improving crop productivity in the field cultivated with ginseng, replacing agrochemicals.

- The population of selected microbe has maintained its relevant proportion for 4 to 9 days during ginseng cultivation. - Plant fungal pathogens were significantly suppressed by the application of B. velezensis CC112 against Rhizoctonia solani (39%) and Pythium ultimum (7%), respectively as compared to the control (no application) without altering bacterial community structure in a ginseng soil.

Keywords
Bacillus
Beneficial microbes
Ginseng rot
Microbial community
Pathogen

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Information
  • Publisher :Korean Society of Soil Science and Fertilizer
  • Publisher(Ko) :한국토양비료학회
  • Journal Title :Korean Journal of Soil Science and Fertilizer
  • Journal Title(Ko) :한국토양비료학회 학회지
  • Volume : 53
  • No :2
  • Pages :128-139
  • Received Date : 2020-03-31
  • Revised Date : 2020-05-07
  • Accepted Date : 2020-05-26
  • DOI :https://doi.org/10.7745/KJSSF.2020.53.2.128
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