Effect of Soaking Seeds in the Bacillus velezensis GH1-13 Solution and Foliar Spray of Chlorella vulgaris on the Seed Potatoes Production

Kyoung-Hee Lee; Jong-Hun Lee; Hyun-Woo Choi; Deog-Bae Lee

doi:10.7745/KJSSF.2021.54.4.413

All Issue

2021 Vol.54, Issue 4 Preview Page Next Page

Original research article

Effect of Soaking Seeds in the Bacillus velezensis GH1-13 Solution and Foliar Spray of Chlorella vulgaris on the Seed Potatoes Production

30 November 2021. pp. 413-422

PDF XML

Abstract

This study was conducted to evaluate effect of microorganisms in the production of seed potatoes based on the mobility rate of disease of common scab and bacterial soft rot and yield of seed potatoes. Two kinds of seed potatoes, the soil culture and the hydroponic were soaked in the culture solution of Bacillus velezensis GH1-13 strains (Soaked GH1-13) and dried at dark room. Seed potatoes were sowed in the field and culture solution of Chorella vulgaris was sprayed on the potato plants at the fifth and the sixth week after sowing. Soaked GH1-13 caused to decrease by 16.5% in the mobility rate of common scab and by 1.0% in that of bacterial soft rot than the control. The chlorella spraying caused to reduce by 12.6% in that of common scab and lowered by 1.1% in that of the bacterial soft rot than the control in the soil cultured seed potatoes. The production population of 30 to 330 grams potatoes per ha, specification of seed potatoes, was more 5,000 ea by the soaked GH1-13 and more 2,666 ea by the chlorella spraying than the control in the soil culture seeds. That was more 555 ea by chlorella spraying than the control in the hydroponic seeds. In the cultivation of soil culture seed potatoes, the soaked GH1-13 caused to increase the yield of seed potatoes by 1,167 kg and spray chlorella caused to increase by 1,405 kg per ha with statistically significant difference. In the cultivation of hydroponic seed potatoes, spraying chlorella caused to increase the production of seed potatoes by 412 kg per ha.

Effect of inoculation of Bacillus velezensis GH1-13 and spraying Chlorella vulgaris on the yield of potato.

Keywords

Bacterial soft rot

Common scab

Plant growth

Seed potatoes

Yield

References

Avis, T.J., V. Gravel, H. Antoun, and R.J. Tweddell. 2008. Multifaceted beneficial effects of rhizosphere microorganisms on plant health and productivity. Soil Biol. Biochem. 40:1733-1740. 10.1016/j.soilbio.2008.02.013

Cha, J.Y., J.W. Kim, B.K. Park, H.J., Jin, S.Y. Kim, and Y.S. Cho. 2008. Isolationand identification of Chlorella sp. CMS-1 the chemical composition of its hot water extract. J. Life Sci. 18:1723-1727. 10.5352/JLS.2008.18.12.1723

Chowdhury, P.S., A. Hartmann, X. Gao, and R. Borriss. 2015. Biocontrol mechanism by root-associated Bacillus amyloliquefaciens FZB42 - a review. Front. Microbiol. 6(780):1-11. 10.3389/fmicb.2015.00780

Guzman, A., M. Gato, and J.M. Calleja. 2001. Antiinflammatory, analgesic and free radical scavenging activities of the marine microalgae Chlorella stigmatophora and Phaeodactylum tricornutum. Phytother. Res. 15:224-230. 10.1002/ptr.71511351357

Guzman, A., M. Gato, M. Lamela, J.M. Gerie-Garabal, and J.M. Calleja. 2003. Anti-inflammatory and immunomodulatory activities of polysaccharide from Chlorella stigmatophora and Phaeodactylum tricornutum. Phytother. Res. 17:665-670. 10.1002/ptr.122712820237

Idris, E.E., D.J. Iglesias, M. Talon, and R. Borriss. 2007. Tryptophan-dependent production of indole-3-acetic acid (IAA) affects level of plant growth promotion by Bacillus amyloliquefaciens FZB42. Mol. Plant-Microbe Interact. 20(6):619-626. 10.1094/MPMI-20-6-061917555270

Insam, H. and M.S.A. Seewald. 2010. Volatile organic compounds (VOCs) in soils. Biol. Fertil. Soils 46(3):199-213. 10.1007/s00374-010-0442-3

Jung, J.C., J.H. Cho, J.S. Im, Y.E. Park, and S.J. Im. 2012. Potato growth survey standards. p. 468-482. Criteria for Research and Analysis of Agricultural Science and Technology The Fifth edition. Rural Development Administration, Suwon, Korea.

Kang, M.S., S.J. Shim, and H.J. Chae. 2004. Chlorella as a functional biomaterial. Korean J. Biotechnol. Bioeng. 19:1-11.

Kim, S.K., C.Y. Ahn, C.M. Ryu, S.M. Lee, B.A. Prasad, N.K. Lee, S.S. Oh, and J.H. Kim. 2016a. Study on the mechanism of Chlorella of suppression of plant disease and improving qualities in organic farming products. p. 102-112. Research Reports of Rural Development Administration, Jeonju, Korea.

Kim, S.Y., H. Song, M.K. Sang, H.-Y. Weon, and J. Song. 2017. The complete genome sequence of Bacillus velezensis strain GH1-13 reveals agriculturally beneficial properties and a unique plasmid. J. Biotechnol. 259:221-227. 10.1016/j.jbiotec.2017.06.120628690133

Kim, S.Y., M.K. Sang, H.-Y. Weon, Y.-A. Jeon, J.H. Ryoo, and J. Song. 2016b. Characterization of multifunctional Bacillus sp. GH1-13. Korean J. Pestic. Sci. 20:189-196. 10.7585/kjps.2016.20.3.189

Lee, S.Y., H.Y. Weon, J.J. Kim, J.H. Han, and W.G. Kim. 2013. Control effect of the mixture of Bacillus amyloliquefaciens M27 and plant extract against cucumber powdery mildew. Korean J. Pestic. Sci. 17(4):435-439. 10.7585/kjps.2013.17.4.435

NAAS (National Academy of Agricultural Science). 2010. Method of soil chemical analysis. Rural Development Administration, Suwon, Korea.

NIAST (National Institute of Agricultural Science and Technology). 2000. Methods of soil and plant analysis. Rural Development Administration, Suwon, Korea.

Ongena, M. and P. Jacques. 2008. Bacillus lipopeptides: Versatileweapons for plant disease biocontrol. Trends Microbiol. 16(3):115-125. 10.1016/j.tim.2007.12.00918289856

Park, K., D. Paul, and W.H. Yeh. 2006. Bacillus vallismortis EXTN-1-mediated growth promotion and disease suppression in rice. Plant Pathol. J. 22(3):278-282. 10.5423/PPJ.2006.22.3.278

Raaijmakers, J.M., I. De Bruijn, O. Nybroe, and M. Ongena. 2010. Natural functions of lipopeptides from Bacillus and Pseudomonas: More than surfactants and antibiotics. FEMS Microbiol. Rev. 34(6):1037-1062. 10.1111/j.1574-6976.2010.00221.x20412310

Santoyo, G., M.D.C. Orozco-Mosqueda, and M. Govindappa. 2012. Mechanisms of biocontrol and plant growth promoting activity in soil bacterial species of Bacillus and Pseudomonas: A review. Biocontrol Sci. Technol. 22:855-872. 10.1080/09583157.2012.694413

Shibata, S., Y. Natori, T. Nishihara, K. Tomisaka, K. Matsumoto, H. Sansawa, and V.C. Nquyen. 2003. Antioxidant and anti-cataract effects of Chlorella on rats with streptozotocin-induced diabetes. J. Nutr. Sci. Vitaminol. 49:334-339. 10.3177/jnsv.49.33414703308

Stirk, W.A., P. Bálint, D. Tarkowská, O. Novák, G. Maróti, K. Ljung, V. Turečková, M. Strnad, V. Ördög, and J. van Staden. 2014. Effect of light on growth and endogenous hormones in Chlorella minutissima (Trebouxiophyceae). Plant Physiol. Biochem. 79:66-76. 10.1016/j.plaphy.2014.03.00524685518

Yoo, H.S., D.C. Jang, J.H. Cho, J.S. Im, and Y.E. Park. 2014. High quality seed potato production and support project of potato production techniques in developing countries. p. 9. Completion Report of the Rural Development Administration, Jeonju, Korea.

Information

Publisher :Korean Society of Soil Science and Fertilizer
Publisher(Ko) :한국토양비료학회
Journal Title :Korean Journal of Soil Science and Fertilizer
Journal Title(Ko) :한국토양비료학회 학회지
Volume : 54
No :4
Pages :413-422
Received Date : 2021-08-25
Revised Date : 2021-09-19
Accepted Date : 2021-11-16
DOI :https://doi.org/10.7745/KJSSF.2021.54.4.413

Korean Journal of Soil Science and Fertilizer ISSN:0367-6315(Print) 2288-2162(Online) 한국토양비료학회 학회지

All Issue