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2019 Vol.52, Issue 2 Preview Page

Original research article

Effect of Large-Scale Cultivation of Bacillus amlyoliquefaciens Y1 Using Fertilizer Based Medium for Control of Citrus Melanose Causing Diaporthe citri
Dong-Ryul Lee1
,
Ei Htwe Maung Chaw1
,
Henry Ajuna1
,
Kil-Yong Kim1∗
1Department of Agricultural and Biological Chemistry, Institute of Environmentally-Friendly Agriculture, Chonnam National University
∗Corresponding Author
31 May 2019. pp. 84-92
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Abstract

The melanose disease causing Diaporthe citri is important pathogen in citrus plants worldwide. Currently, there are few researches related to biological control of melanose disease. In this study, we investigated control of Diaporthe citri by Bacillus amyloliquefaciens Y1 isolated from the soil. Low-cost fertilizer based PB medium and large-scale fermenter have been developed for field application of biological control. We prove that Y1 can be successfully grown in large-scale fermenters using PB medium without contamination. In addition, Y1 bacterial culture filtrates (BCF) of large-scale cultivation in PB medium (LP-Y1), present better growth inhibition of D. citri than small-scale cultivation in TSB medium (ST-Y1). Microscopic observations also showed that pathogen mycelial morphology was significantly affected by BCF, presumably due to metabolites produced by Y1. Finally, in pot experiment, pre-treatment with fungicide and Y1 to leaves significantly reduced disease incidence compared to the control group. This study showed that Y1 incubated with fertilizer based medium inhibited the occurrence of D. citri and could be grown in large scale field conditions without contamination as well as cost optimization.

Mycelial growth inhibition of D. citri by different bacterial culture filtrates concentrations of the strain Y1 from the large-scale in PB medium (LP-Y1), the small-scale in TSB medium (ST-Y1)

Keywords
Bacillus amyloliquefaciens Y1
Large-scale cultivation
Fertilizer based medium
Diaporthe citri

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Information
  • Publisher :Korean Society of Soil Science and Fertilizer
  • Publisher(Ko) :한국토양비료학회
  • Journal Title :Korean Journal of Soil Science and Fertilizer
  • Journal Title(Ko) :한국토양비료학회 학회지
  • Volume : 52
  • No :2
  • Pages :84-92
  • Received Date : 2019-01-16
  • Revised Date : 2019-05-17
  • Accepted Date : 2019-05-27
  • DOI :https://doi.org/10.7745/KJSSF.2019.52.2.084
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